On chip CTC staining, detection

Human PBMC spiked with MCF was loaded into SIEVEWELL. After PFA fixation on chip, mixture of Alexa 488 labelled anti pan-CK antibody, Alexa594 labelled anti CD45 antibody and DAPI was added. After 30 minutes incubation, cells were washed with PBS. Image was taken Leica DMI6000B.

A patient sample enriched using CellSearch system was stained on a Sievewell chip.

Data provided by Professor Hans Neubauer
Department of Obstetrics and Gynaecology, University Hospital and Medical Faculty of the Heinrich Heine University Düsseldorf, Düsseldorf, Germany