MCF7をスパイクしたヒトPBMCをSIEVEWELLにロードした。デバイス内でPFA固定したのち、Alexa 488標識抗pan-CK抗体とAlexa594標識CD45抗体、DAPIのカクテル溶液を添加し、反応させた。PBSで洗浄したのち、ライカDMI6000B倒立蛍光顕微鏡にて撮影した。


Data provided by Professor Hans Neubauer
Department of Obstetrics and Gynaecology, University Hospital and Medical Faculty of the Heinrich Heine University Düsseldorf, Düsseldorf, Germany

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