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CTCのデバイス内での染色

免疫染色は、細胞の固定、透過処理、ブロッキング、抗体反応、洗浄といった複数のステップが必要ですが、染色操作の過程で細胞が失われることがあります。SIEVEWELLを使用すると、デバイス内ですべてのステップを行うことができます。染色時の試薬は貫通孔を通過しますが、細胞はフィルター上に残るため、免疫染色時の細胞のロスを最小限にすることができます。


MCF7をスパイクしたヒトPBMCをSIEVEWELLにロードした。デバイス内でPFA固定したのち、Alexa 488標識抗pan-CK抗体とAlexa594標識CD45抗体、DAPIのカクテル溶液を添加し、反応させた。PBSで洗浄したのち、ライカDMI6000B倒立蛍光顕微鏡にて撮影した。


CellSearchシステムで濃縮した患者血液由来サンプルをSIEVEWELLにロードし、CTCの染色と検出を行った事例。

Data provided by Professor Hans Neubauer
Department of Obstetrics and Gynaecology, University Hospital and Medical Faculty of the Heinrich Heine University Düsseldorf, Düsseldorf, Germany

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