Blood Separation
This example shows the enrichment of lymphocytes from small volume of mouse whole blood.
![](http://www.sievewell.com/media/2020/10/Mouse_blood_loaded-1024x768.jpg)
50 μL of mouse whole blood was diluted 1:10 with PBS. Diluted blood was loaded into SIEVEWELL.
![](http://www.sievewell.com/media/2020/10/Mouse_blood_washed-1024x768.jpg)
RBCs were removed from pores of each well by aspiration. 2 times washing step with PBS were performed to remove remaining RBCs.
![](http://www.sievewell.com/media/2020/10/Mouse_blood_stained-1024x771.jpg)
Calcein AM Fluorescent Dye was added into chip. After 15 minutes incubation, cells were washed with PBS. Stained live lymphocytes were visualized with microscope.