Step 3 Staining
3-1
Aspirate all buffer.
3-2
Add reagent.
3-3
Aspirate 0.2 mL.
Incubate at required temperature for required time
3-4
Add buffer.
3-5
Aspirate
Repeat step 3-4 and 3-5 two times.
3-6
Add 1 mL of PBS.
Aspirate all buffer.
Add reagent.
Aspirate 0.2 mL.
Incubate at required temperature for required time
Add buffer.
Aspirate
Repeat step 3-4 and 3-5 two times.
Add 1 mL of PBS.