Mechanism of Single Cell Capture

Arraying cells is an important step for single cell analysis. For example, cell overlap is unfavourable for single cell imaging and isolation. Microcavity arrays are used to capture single cells. In general, the distribution of cells in a cavity is by sedimentation and the single cell rate follows a Poisson distribution. Its single cell capture rate is relatively low, especially when loading cells in higher quantities.

Two pores are positioned at the bottom of the nanowell.

Micro-gap situated under the nanowell structure.

A directional liquid flow can be created by aspirating liquid.

The cells follow the liquid flow and are captured in the nanowell.

When a cell enters a nanowell, it blocks the pores, reducing the flow of liquid through that nanowell. Other cells are then redirected to other empty nanowells.

This mechanism allows a higher single cell rate than a Poisson distribution.

Easy to use. Fluidic system or instrument is not required.

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