On-Chip Neural Differentiation of PC12 Cells

SIEVEWELL™ 20 μm was coated with Cellmatrix® Type IV (Collagen Type IV, Nitta Gelatin Inc). Rat pheochromocytoma cell line PC12 cells were loadetd into SIEVEWELL™ 20 μm. Cells were cultured with RPMI 1640/10%horse serum/5% fetal bovine serum containing 10 ng/μL NGF. After 7 days, cells were fixed with PFA, permiabilized with 0.05% Tween 20/PBS, blocked wtih 1%BSA/PBS, stained wtih mouse anti-rat Tubulin β3 (TUBB3) antibody (Clone, TUJ1) followed by staining with Alexa Fluor Plus 488 labelled anti mouse IgG antibody and DAPI. Images were taken with THUNDER Imaging Systems (Leica Microsystems).

Privacy Settings
We use cookies to enhance your experience while using our website. If you are using our Services via a browser you can restrict, block or remove cookies through your web browser settings. We also use content and scripts from third parties that may use tracking technologies. You can selectively provide your consent below to allow such third party embeds. For complete information about the cookies we use, data we collect and how we process them, please check our Privacy Policy
Consent to display content from - Youtube
Consent to display content from - Vimeo
Google Maps
Consent to display content from - Google
Consent to display content from - Spotify
Sound Cloud
Consent to display content from - Sound