On-chip Neural differentiation of PC12 cells

On-Chip Neural Differentiation of PC12 Cells SIEVEWELL™ 20 μm was coated with Cellmatrix® Type IV (Collagen Type IV, Nitta Gelatin Inc). Rat pheochromocytoma cell line PC12 cells were loadetd into SIEVEWELL™ 20 μm. Cells were cultured with RPMI 1640/10%horse serum/5% fetal bovine serum containing 10 ng/μL NGF. After 7 days, cells were fixed with PFA,...

Culture of Cardiomyocytes

Culture of Cardiomyocytes and Calcium Imaging CarmyA-GCaMP (Myoridge Co. Ltd.) is human iPS cell-derived cardiomyocytes expressing GCaMP, a calcium sensor. This video shows culture of cardiomyocytes and calcium cycling. Calcium cycling of single cell suspension state on 20 μm and 50 μm SIEVEWELL™ Beating cardiomyocyte spheroid on 50 μm SIEVEWELL™ Calcium imaging of cardiomyocyte...